Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli.

نویسندگان

  • T W North
  • G L Hansen
  • Y Zhu
  • J A Griffin
  • C K Shih
چکیده

Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.

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عنوان ژورنال:
  • Antimicrobial agents and chemotherapy

دوره 38 2  شماره 

صفحات  -

تاریخ انتشار 1994